3CWK

Crystal Structure of the R132K:Y134F:R111L:T54V:L121E Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-trans-Retinoic Acid at 1.57 Angstroms Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 32-ID
Synchrotron site	APS
Beamline	32-ID
Temperature [K]	77
Detector technology	CCD
Collection date	2004-03-13
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	45.787, 44.860, 77.865
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.470 - 1.600
R-factor	0.125
Rwork	0.120
R-free	0.16700
Structure solution method	RIGID BODY REFINEMENT
RMSD bond length	0.020
RMSD bond angle	1.881
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC (5.2.0005)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.050	0.157
Number of reflections	21826
<I/σ(I)>	27.28
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M MES, 30% (W/V) PEG 5000, 0.2 M AMMONIUM SULFATE, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K