3CW5

E. coli Initiator tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Collection date	2006-11-25
Wavelength(s)	0.931
Spacegroup name	P 64 2 2
Unit cell lengths	93.790, 93.790, 219.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.918 - 3.100
R-factor	0.244
Rwork	0.241
R-free	0.27100
Starting model (for MR)	E. coli tRNAfMet from 2FMT
RMSD bond length	0.009
RMSD bond angle	1.735
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.918	76.250	3.270
High resolution limit [Å]	3.100	9.800	3.100
Rmerge	0.099	0.053	0.476
Total number of observations		3185	2894
Number of reflections	10063
<I/σ(I)>	3.8	5.2	1.5
Completeness [%]	91.4	98.4	59.8
Redundancy	7.9	7.5	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	292	2.0 M Ammonium Sulphate, pH 4.6, vapor diffusion, temperature 292K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	(NH4)2SO4
2	1	2	(NH4)2SO4