3CVF
Crystal Structure of the carboxy terminus of Homer3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-09 |
| Detector | Quantum-4 ADSC |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 172.029, 172.029, 66.446 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.730 - 2.900 |
| R-factor | 0.25391 |
| Rwork | 0.252 |
| R-free | 0.28698 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cve |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.312 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.078 | 0.044 | 0.255 |
| Number of reflections | 25036 | ||
| <I/σ(I)> | 14.5 | ||
| Completeness [%] | 99.4 | 98.1 | 98.1 |
| Redundancy | 7.2 | 8.2 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.6 | 297 | 0.1 M Tris, 4.2 M NaCl, 10% glycerol, pH 7.6, hanging drop, temperature 297K |
