3CUY
Crystal Structure of GumK mutant D157A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.433 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 121.726, 121.726, 172.557 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.990 - 2.300 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hy7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.622 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.220 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.171 | 0.281 |
| Number of reflections | 33977 | |
| <I/σ(I)> | 3.1 | 6.7 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 7.6 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 8.2 | 293 | 0.1 M Tris-HCl, 35% (w/v) PEG 3350, 0.2 M Li2SO4, 0.1 M LiCl, pH 8.2, hanging drop, temperature 293K |
