3CUQ
Integrated structural and functional model of the human ESCRT-II complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-04-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.162, 89.153, 91.437 |
| Unit cell angles | 90.00, 101.52, 90.00 |
Refinement procedure
| Resolution | 44.800 - 2.610 |
| R-factor | 0.24074 |
| Rwork | 0.238 |
| R-free | 0.29804 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.635 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 30680 | |
| <I/σ(I)> | 33.1 | 3.13 |
| Completeness [%] | 91.4 | 61.1 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 300 | 5% PEG4000, 15% Glycerol, 100mM Na-Acetate, pH4.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
