3CUP
Crystal structure of the MHC class II molecule I-Ag7 in complex with the peptide GAD221-235
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 96 |
| Detector technology | CCD |
| Collection date | 1999-06-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.832, 55.832, 338.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.450 - 3.090 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1es0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.638 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.449 | 3.150 |
| High resolution limit [Å] | 3.088 | 3.100 |
| Number of reflections | 10586 | |
| <I/σ(I)> | 10.6 | 1.8 |
| Completeness [%] | 98.7 | 99.4 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295 | 0.1M Hepes pH 7.5, 28% PEG 8000, 2% Jeffamine 900, VAPOR DIFFUSION, SITTING DROP, temperature 295K, pH 7.50 |
