3CU7
Human Complement Component 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 31 |
| Unit cell lengths | 144.260, 144.260, 241.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.286 - 3.105 |
| R-factor | 0.2379 |
| Rwork | 0.236 |
| R-free | 0.28050 |
| Structure solution method | MOLECULAR REPLACEMENT and SAD |
| Starting model (for MR) | 2B39 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.846 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.040 | 0.570 |
| Number of reflections | 98600 | |
| <I/σ(I)> | 13.5 | 1.3 |
| Completeness [%] | 96.9 | 86 |
| Redundancy | 1.36 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 100mM NaCl, 1.5-3mM MES pH 5.5, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
