3CU5
Crystal structure of a two component transcriptional regulator AraC from Clostridium phytofermentans ISDg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.326, 87.038, 52.697 |
| Unit cell angles | 90.00, 106.22, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.189 |
| Rwork | 0.183 |
| R-free | 0.30000 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.098 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.101 | 0.061 | 0.407 |
| Number of reflections | 16975 | ||
| <I/σ(I)> | 7.7 | ||
| Completeness [%] | 85.8 | 99.3 | 36.4 |
| Redundancy | 3.3 | 3.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 1.8 M Tri-ammonium citrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
