3CU1
Crystal Structure of 2:2:2 FGFR2D2:FGF1:SOS complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-07-08 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.929, 110.375, 74.699 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.950 - 2.600 |
| R-factor | 0.18522 |
| Rwork | 0.180 |
| R-free | 0.27680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.053 |
| RMSD bond angle | 3.136 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.069 | 0.038 | 0.281 |
| Number of reflections | 21046 | ||
| <I/σ(I)> | 20.5 | ||
| Completeness [%] | 93.3 | 91.6 | 67.9 |
| Redundancy | 3.9 | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 12% PEG3350, 4% Tacsimate, pH 7.0, vapor diffusion, hanging drop, temperature 298.0K |
