3CT6
Crystal structure of DhaM of L. lactis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.600, 62.400, 69.700 |
| Unit cell angles | 90.00, 99.60, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.100 |
| R-factor | 0.1468 |
| R-free | 0.17690 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.033 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE (2.08) |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.136 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.069 | 0.253 |
| Number of reflections | 97245 | |
| <I/σ(I)> | 17.41 | 4.4 |
| Completeness [%] | 93.0 | 90.77 |
| Redundancy | 2.489 | 2.218 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.8 | 289 | 200 mM NaNO3, 20 % PEG 3350, pH 2.8, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
