3CRV
XPD_Helicase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-26 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.12710, 0.97931, 0.97899, 0.91837 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.542, 70.222, 144.293 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.580 - 2.000 |
| R-factor | 0.227 |
| Rwork | 0.222 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.580 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.410 | |
| Number of reflections | 37102 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 99.0 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.4M Citrate pH 5.6 15-20% PEG 4000 10% Isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
