3CR3

Structure of a transient complex between Dha-kinase subunits DhaM and DhaL from Lactococcus lactis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97885,0.97823,0.97240
Spacegroup name	P 21 21 2
Unit cell lengths	74.133, 146.842, 58.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.230 - 2.100
R-factor	0.22168
Rwork	0.219
R-free	0.26300
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.215
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	147.442	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.047	0.161
Number of reflections	37982
<I/σ(I)>	22.2	8.1
Completeness [%]	99.8	24.4
Redundancy	4.9	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.1 M Tris/HCl, 0.2 M LiSO4, 36 % PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K