3CR1
crystal structure of a minimal, mutant, all-RNA hairpin ribozyme (A38C, A-1OMA) grown from MgCl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-06-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.640, 93.640, 133.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.890 - 2.250 |
| Rwork | 0.219 |
| R-free | 0.24600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2oue |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.890 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.040 | 0.541 |
| Number of reflections | 16729 | |
| <I/σ(I)> | 25.8 | 4.4 |
| Completeness [%] | 98.2 | 99.7 |
| Redundancy | 11.3 | 11.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG2000 MME, lithium sulfate, magnesium chloride, spermidine, sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG2000 MME | ||
| 10 | 1 | 2 | sodium cacodylate | ||
| 2 | 1 | 1 | lithium sulfate | ||
| 3 | 1 | 1 | magnesium chloride | ||
| 4 | 1 | 1 | spermidine | ||
| 5 | 1 | 1 | sodium cacodylate | ||
| 6 | 1 | 2 | PEG2000 MME | ||
| 7 | 1 | 2 | lithium sulfate | ||
| 8 | 1 | 2 | magnesium chloride | ||
| 9 | 1 | 2 | spermidine |
