3CQZ

Crystal structure of 10 subunit RNA polymerase II in complex with the inhibitor alpha-amanitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	I 2 2 2
Unit cell lengths	122.510, 222.480, 374.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.202
Rwork	0.200
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k83
RMSD bond length	0.015
RMSD bond angle	1.827
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Number of reflections	124441

Crystallization Conditions

crystal ID	method	pH	temperature	details
1