3CQO
Crystal structure of a f-lectin (fucolectin) from morone saxatilis (striped bass) serum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 88.135, 88.135, 230.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.150 - 2.320 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k12 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.150 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.110 | 0.473 |
| Number of reflections | 38943 | |
| <I/σ(I)> | 26.6 | 4.2 |
| Completeness [%] | 96.8 | 90.2 |
| Redundancy | 9.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | PEG 2K MME 25 % W/V, PH 9.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K, pH 9.00 |
