3CQ9
Crystal structure of the lp_1622 protein from Lactobacillus plantarum. Northeast Structural Genomics Consortium target LpR114
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97885, 0.97938, 0.96785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.784, 194.031, 52.093 |
| Unit cell angles | 90.00, 115.34, 90.00 |
Refinement procedure
| Resolution | 44.670 - 2.200 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.20400 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.076 |
| Number of reflections | 88438 | |
| <I/σ(I)> | 32.9 | 19.3 |
| Completeness [%] | 95.1 | 79.7 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 18% PEG 3350, 0.2M Ammonium sulfate, 0.1M Sodium acetate pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
