3CQ5
Histidinol-phosphate aminotransferase from Corynebacterium glutamicum in complex with PMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.939 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.273, 85.531, 89.431 |
| Unit cell angles | 90.00, 93.65, 90.00 |
Refinement procedure
| Resolution | 27.000 - 1.800 |
| R-factor | 0.19313 |
| Rwork | 0.192 |
| R-free | 0.21916 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.445 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.456 |
| Number of reflections | 133625 | |
| <I/σ(I)> | 15.9 | 2.7 |
| Completeness [%] | 98.5 | 97.7 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.7M (NH4)2SO4, 0.2M Li2SO4, 0.1M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
