3CPL
Crystal Structure of H-2Db in complex with a variant M6A of the NP366 peptide from influenza A virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.710, 69.700, 72.380 |
| Unit cell angles | 99.95, 111.04, 110.48 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.227 |
| Rwork | 0.220 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yn7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.422 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.094 | 0.273 |
| Number of reflections | 30549 | 3336 |
| <I/σ(I)> | 13.69 | 5 |
| Completeness [%] | 95.0 | 93.9 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M potassium thiocianate, 30% PEG 2000, vapor diffusion, hanging drop, temperature 293K |
