3CPH
Crystal structure of Sec4 in complex with Rab-GDI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9816 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 131.900, 131.900, 217.562 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.2202 |
| Rwork | 0.218 |
| R-free | 0.26763 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ukv chain G |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 48896 | |
| Completeness [%] | 99.3 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 12% PEG MME 2000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
