3CP8
Crystal structure of GidA from Chlorobium tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97955 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.505, 71.836, 171.934 |
| Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
| Resolution | 44.850 - 3.200 |
| R-factor | 0.25568 |
| Rwork | 0.254 |
| R-free | 0.27809 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cp2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.185 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.400 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 48825 | |
| <I/σ(I)> | 26.8 | 3.5 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 13-15% PEG 8000, 200mM ammonium sulfate, 100mM TRIS, 78mM HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
