3CP7
Crystal structure of a thermostable serine protease AL20 from extremophilic microoganism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-01 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.076 |
Spacegroup name | H 3 |
Unit cell lengths | 92.260, 92.260, 137.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.180 - 1.390 |
R-factor | 0.17887 |
Rwork | 0.178 |
R-free | 0.19904 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p3e |
RMSD bond length | 0.008 |
RMSD bond angle | 1.314 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.800 | 1.440 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.049 | 0.193 |
Number of reflections | 86325 | |
<I/σ(I)> | 14.5 | 3.5 |
Completeness [%] | 98.0 | 89.4 |
Redundancy | 3.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 294 | 2.9M sodium formate, 0.1M sodium citrate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 294K |