3COC
Crystal Structure of D115A mutant of Bacteriorhodopsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1271 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.362, 108.966, 56.432 |
Unit cell angles | 90.00, 113.72, 90.00 |
Refinement procedure
Resolution | 29.710 - 2.310 |
Rwork | 0.221 |
R-free | 0.27500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 90.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.130 | 0.085 | 0.365 |
Number of reflections | 20963 | ||
<I/σ(I)> | 9.6 | ||
Completeness [%] | 93.8 | 99.2 | 84 |
Redundancy | 3 | 3.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 310 | 1.4 M NaH2PO4 (pH 3.7), 0.8 M NaH2PO4 (pH 4.5), and 0.12 M hexanediol, bicelle method, temperature 310K |