3COB
Structural Dynamics of the Microtubule binding and regulatory elements in the Kinesin-like Calmodulin binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-11-18 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.152, 84.907, 89.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sdm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 37114 | |
| <I/σ(I)> | 13.7 | 3.1 |
| Completeness [%] | 95.5 | 84.8 |
| Redundancy | 7.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.1 | 298 | 20% PEG 3350, 0.2M di-Sodium hydrogen Phosphate, pH 9.1, temperature 298.0K |
