3CNR
Crystal Structure of PilZ (XAC1133) from Xanthomonas axonopodis pv citri
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978294, 0.978581, 0.953724 |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.125, 62.125, 83.543 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 1.900 |
| R-factor | 0.19285 |
| Rwork | 0.190 |
| R-free | 0.24345 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.547 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.086 | 0.601 |
| Number of reflections | 13687 | |
| <I/σ(I)> | 24.7 | 1.74 |
| Completeness [%] | 99.0 | 92.1 |
| Redundancy | 10.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 24% PEG 4000, 0.1 M Tris-HCl, 0.2 M magnesium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
