3CNK
Crystal Structure of the dimerization domain of human filamin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-12 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.845, 94.642, 40.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.440 - 1.650 |
| R-factor | 0.19347 |
| Rwork | 0.192 |
| R-free | 0.21855 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v05 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.054 | 0.029 | 0.332 |
| Rmeas | 0.054 | ||
| Total number of observations | 167922 | ||
| Number of reflections | 27127 | ||
| <I/σ(I)> | 31.708 | ||
| Completeness [%] | 97.5 | 99.4 | 99.3 |
| Redundancy | 6.2 | 6.4 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 293 | 0.1M Tris, 30% PEG 3350, 0.2M Lithium Sulfate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8.20 |
