3CNJ
Cholesterol oxidase from Streptomyces sp. F359W mutant (0.95A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-28 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.280, 72.870, 62.950 |
| Unit cell angles | 90.00, 105.11, 90.00 |
Refinement procedure
| Resolution | 46.670 - 0.950 |
| Rwork | 0.140 |
| R-free | 0.17500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1mxt |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 0.980 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.047 | 0.480 |
| Number of reflections | 270322 | |
| <I/σ(I)> | 11.2 | 1.9 |
| Completeness [%] | 96.3 | 67.6 |
| Redundancy | 4.22 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | PEG 8000, MANGANESE SULFATE, CACODYLATE (PH 5.2), VAPOR DIFFUSION, HANGING DROP, temperature 291K |
