3CMU
Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Collection date | 2006-10-01 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 156.000, 156.000, 211.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 4.200 |
| R-factor | 0.276 |
| Rwork | 0.275 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.441 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 4.200 |
| Number of reflections | 16125 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | RecA6 fusion protein was incubated with a 3-fold molar excess of ssDNA (dT)18 in the original protein buffer supplemented with 2 mM ADP, 10 mM MgCl2 and 8 mM AlF4, pH 6.0. Crystals of the RecA6-(ADP-AlF4-Mg)6-(dT)18 complex were grown from 50 mM Hepes-Na+, 1.5% (w/v) polyethylene glycol (PEG) 3350, 4% (w/v) polyvinylpyrrolidone K15 (PVP K15), 25% (v/v) 2-methyl-2,4-pentandiol (MPD), 10 mM DTT, pH 7.5. |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ADP | ||
| 10 | 1 | 2 | PEG 3350 | ||
| 11 | 1 | 2 | PVP K15 | ||
| 12 | 1 | 2 | MPD | ||
| 13 | 1 | 2 | DTT | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | AlF4 | ||
| 4 | 1 | 1 | Hepes-Na+ | ||
| 5 | 1 | 1 | PEG 3350 | ||
| 6 | 1 | 1 | PVP K15 | ||
| 7 | 1 | 1 | MPD | ||
| 8 | 1 | 1 | DTT | ||
| 9 | 1 | 2 | Hepes-Na+ |
