3CME
The Structure of CA and CCA-PHE-CAP-BIO Bound to the Large Ribosomal Subunit of Haloarcula Marismortui
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 210.786, 297.778, 572.588 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.820 - 2.950 |
Rwork | 0.198 |
R-free | 0.25500 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.261 | |
Number of reflections | 369334 | |
<I/σ(I)> | 3.7 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 292 | PEG 6000, KCL, NH4CL, MGCL2, KACETATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
10 | 1 | 2 | KACETATE | ||
2 | 1 | 1 | KCL | ||
3 | 1 | 1 | NH4CL | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 1 | KACETATE | ||
6 | 1 | 2 | PEG 6000 | ||
7 | 1 | 2 | KCL | ||
8 | 1 | 2 | NH4CL | ||
9 | 1 | 2 | MGCL2 |