3CM6
Crystal structure of cell-death related nuclease 4 (CRN-4) bound with Er
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 130.279, 157.795, 65.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| Rwork | 0.225 |
| R-free | 0.26516 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cg7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 43082 | |
| <I/σ(I)> | 19.6 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.9 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.2M sodium formate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
