3CLU
Crystal structure of the R236K mutant from Methylophilus methylotrophus ETF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 |
| Unit cell lengths | 116.482, 116.482, 84.281 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.000 - 1.800 |
| R-factor | 0.16963 |
| Rwork | 0.168 |
| R-free | 0.20395 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o97 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.541 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 58203 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 99.0 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | limited digest with trypsin, 2.0 M sodium phosphate, pH 6.2, VAPOR DIFFUSION |
