3CLQ
Crystal structure of a conserved protein of unknown function from Enterococcus faecalis V583
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97951, 0.97965 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 104.159, 61.613, 164.544 |
Unit cell angles | 90.00, 103.94, 90.00 |
Refinement procedure
Resolution | 43.030 - 2.500 |
R-factor | 0.18605 |
Rwork | 0.183 |
R-free | 0.23972 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.546 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.030 | 2.570 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.117 | 0.559 |
Number of reflections | 62694 | |
<I/σ(I)> | 22.9 | 1.88 |
Completeness [%] | 88.8 | 54.9 |
Redundancy | 4.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M NaCl, 0.1M Tris-HCl, 25% w/v PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |