3CLC
Crystal Structure of the Restriction-Modification Controller Protein C.Esp1396I Tetramer in Complex with its Natural 35 Base-Pair Operator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9322 |
Spacegroup name | P 65 |
Unit cell lengths | 104.480, 104.480, 139.290 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.060 - 2.800 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.328 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 | |
High resolution limit [Å] | 2.800 | 15.000 | 2.800 |
Rmerge | 0.043 | 0.013 | 0.393 |
Number of reflections | 21198 | 71 | 2103 |
<I/σ(I)> | 33.66 | 91 | 4.8 |
Completeness [%] | 98.5 | 49 | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 50 mM MES, 40 mM MgCl2, 25% MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MES | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | MES | ||
6 | 1 | 2 | MgCl2 | ||
7 | 1 | 2 | MPD | ||
8 | 1 | 2 | H2O |