3CL1
M. loti cyclic-nucleotide binding domain, cyclic-GMP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Collection date | 2004-04-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.202, 79.594, 49.984 |
| Unit cell angles | 90.00, 99.11, 90.00 |
Refinement procedure
| Resolution | 30.980 - 2.400 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.309 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.326 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.081 | 0.155 |
| Number of reflections | 15482 | |
| <I/σ(I)> | 2 | 3 |
| Completeness [%] | 87.0 | 64.9 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP |
