3CKQ
Crystal Structure of a Mycobacterial Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.0053 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.025, 87.025, 103.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.130 - 3.000 |
| R-factor | 0.22506 |
| Rwork | 0.224 |
| R-free | 0.25353 |
| Structure solution method | Rigid body refinement |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.923 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.130 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 7931 | |
| <I/σ(I)> | 7 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1 M Ammonium sulphate, 0.1 M HEPES pH 7.0, 20 mM Manganese chloride, 1 mM Sodium thiosulphate, 100 mM uridine-5 -diphosphate-glucose, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
