3CJH
Tim8-Tim13 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.655, 56.303, 59.837 |
| Unit cell angles | 89.18, 89.65, 60.30 |
Refinement procedure
| Resolution | 19.940 - 2.600 |
| R-factor | 0.24691 |
| Rwork | 0.244 |
| R-free | 0.28884 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.320 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 0.378 |
| Number of reflections | 19026 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 96.0 | 94.1 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 298 | 10 mM Tris, pH 8.0, 10 mM NaCl, 3% 2-methyl-2,4-pentanediol (MPD), VAPOR DIFFUSION, SITTING DROP, temperature 298K |
