3CIK
Human GRK2 in Complex with Gbetagamma subunits
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-02-23 |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.736, 73.604, 122.914 |
| Unit cell angles | 90.00, 115.22, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.750 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.26900 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 5.920 | 2.750 |
| Rmerge | 0.101 | 0.056 | 0.545 |
| Number of reflections | 38796 | ||
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 98.8 | 94.3 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 277 | 200 mM NaCl, 5% ethylene glycol, 6.3% PEG 3350, pH 5.25, vapor diffusion, hanging drop, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
