3CHS

Crystal structure of leukotriene A4 hydrolase in complex with (2S)-2-amino-5-[[4-[(2S)-2-hydroxy-2-phenyl-ethoxy]phenyl]amino]-5-oxo-pentanoic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-11
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 2
Unit cell lengths	67.618, 133.266, 83.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.550
R-factor	0.21306
Rwork	0.211
R-free	0.24753
Structure solution method	OTHER
RMSD bond length	0.008
RMSD bond angle	1.054
Phasing software	CNX
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.960
High resolution limit [Å]	2.550
Number of reflections	25287
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	8	298	PEG 8000, SODIUM ACETATE, IMIDEAZOLE PH 6.8, YBCL2, BESTATIN, PH 8.0, LIQUID DIFFUSION, TEMPERATURE 298K, pH 8.00