3CHM
Crystal structure of PCI domain from A. thaliana COP9 signalosome subunit 7 (CSN7)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-11-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 57.094, 85.796, 72.343 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.900 - 1.500 |
R-factor | 0.16559 |
Rwork | 0.164 |
R-free | 0.19996 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.540 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 28844 | |
<I/σ(I)> | 47.9 | 6.7 |
Completeness [%] | 99.5 | 97.1 |
Redundancy | 8.9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 292 | 23% w/v PEG 8000, 0.1M sodium cacodylate (pH 7.2), 0.06-0.08M Mg-acetate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 292 | 23% w/v PEG 8000, 0.1M sodium cacodylate (pH 7.2), 0.06-0.08M Mg-acetate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 292 | 23% w/v PEG 8000, 0.1M sodium cacodylate (pH 7.2), 0.06-0.08M Mg-acetate tetrahydrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K |