3CHL

Crystal Structure of Alpha-14 Giardin with magnesium bound

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Detector technology	CCD
Collection date	2007-05-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979, 0.91216, 0.9793, 0.9795
Spacegroup name	H 3
Unit cell lengths	133.859, 133.859, 69.668
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.900
R-factor	0.201
Rwork	0.199
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.465
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE (2.09)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	66.960
High resolution limit [Å]	1.900
Number of reflections	36608
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	273	100 mM magnesium chloride, 100 mM sodium citrate pH 5.0, and 15% (w/v) PEG 4000 , VAPOR DIFFUSION, SITTING DROP, temperature 273K