3CH2
Crystal Structure Analysis of SERA5E from plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 103.590, 103.590, 72.132 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.460 - 1.800 |
| R-factor | 0.1677 |
| Rwork | 0.166 |
| R-free | 0.19758 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ch3 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.459 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.093 | 0.048 | 0.809 |
| Number of reflections | 26794 | ||
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 4.2 | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 18% PEG 3350, 0.2M CaCl2, unbuffered, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
