3CGY
Crystal Structure of Salmonella Sensor Kinase PhoQ catalytic domain in complex with radicicol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-27 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.923, 63.759, 87.772 |
| Unit cell angles | 90.00, 121.41, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| Rwork | 0.246 |
| R-free | 0.29900 |
| Starting model (for MR) | 1id0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.644 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.010 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.073 | 0.465 |
| Number of reflections | 12655 | |
| <I/σ(I)> | 9.4 | 2.2 |
| Completeness [%] | 98.1 | 97.9 |
| Redundancy | 3.6 | 3.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 27.5% PEG 4000, 0.1M MOPS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
