3CGL
Crystal Structure and Raman Studies of dsFP483, a Cyan Fluorescent Protein from Discosoma striata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.202, 78.269, 188.566 |
| Unit cell angles | 90.00, 91.35, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.090 |
| Rwork | 0.185 |
| R-free | 0.21668 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1G7K |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.520 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.100 |
| Rmerge | 0.070 | |
| Number of reflections | 90778 | |
| <I/σ(I)> | 21.8 | 5.4 |
| Completeness [%] | 94.8 | 67.5 |
| Redundancy | 7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 277 | 100 mM HEPES pH 7.9, 300 mM NaCl, 200 mM calcium acetate, 16% v/v PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
