3CGL

Crystal Structure and Raman Studies of dsFP483, a Cyan Fluorescent Protein from Discosoma striata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	111.202, 78.269, 188.566
Unit cell angles	90.00, 91.35, 90.00

Refinement procedure

Resolution	40.000 - 2.090
Rwork	0.185
R-free	0.21668
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB code 1G7K
RMSD bond length	0.011
RMSD bond angle	1.520
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.180
High resolution limit [Å]	2.090	2.100
Rmerge	0.070
Number of reflections	90778
<I/σ(I)>	21.8	5.4
Completeness [%]	94.8	67.5
Redundancy	7	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	277	100 mM HEPES pH 7.9, 300 mM NaCl, 200 mM calcium acetate, 16% v/v PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K