3CG4
Crystal structure of response regulator receiver domain protein (CheY-like) from Methanospirillum hungatei JF-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-03-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 65 |
| Unit cell lengths | 69.454, 69.454, 49.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.610 |
| R-factor | 0.20213 |
| Rwork | 0.201 |
| R-free | 0.22596 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.312 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.630 | 1.690 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.104 | 0.950 |
| Number of reflections | 17659 | |
| <I/σ(I)> | 11.6 | 3 |
| Completeness [%] | 99.2 | 96.3 |
| Redundancy | 17.4 | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 100mM Bis-Tris pH 6.5, 25% PEG 3350, 200mM Sodium chloride, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
