3CFI
Nanobody-aided structure determination of the EPSI:EPSJ pseudopilin heterdimer from Vibrio Vulnificus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-02 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.12709 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.720, 67.243, 128.255 |
| Unit cell angles | 96.54, 91.62, 90.20 |
Refinement procedure
| Resolution | 19.970 - 2.580 |
| R-factor | 0.23186 |
| Rwork | 0.229 |
| R-free | 0.27899 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.849 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.680 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.129 | 0.591 |
| Number of reflections | 49095 | |
| <I/σ(I)> | 14.78 | 2.72 |
| Completeness [%] | 90.0 | 71 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 25% PEG 3350, 0.1M Ammonium Sulfate, 0.1M Bis-Tris, pH 5.5, vapor diffusion, sitting drop, temperature 298K |
