3CFB
High-resolution structure of blue fluorescent antibody EP2-19G2 in complex with stilbene hapten at 100K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-10 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.707, 61.015, 92.246 |
| Unit cell angles | 90.00, 116.65, 90.00 |
Refinement procedure
| Resolution | 42.030 - 1.600 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fl3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.597 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0017) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 121549 | |
| <I/σ(I)> | 30.3 | 1.9 |
| Completeness [%] | 97.4 | 96.2 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 20% PEG 4000, 0.2M KSCN, PH 5.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K, pH 5.50 |
