3CDU
Crystal structure of coxsackievirus B3 RNA-dependent RNA polymerase (3Dpol) in complex with a pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.405, 74.405, 285.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.980 - 2.100 |
| R-factor | 0.1848 |
| Rwork | 0.178 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ra6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.157 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER-TNT (2.3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.300 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 40919 | |
| <I/σ(I)> | 25.4 | 6.2 |
| Completeness [%] | 90.6 | 52.6 |
| Redundancy | 4.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 293 | 2M ammonium sulfate, 0.1M CAPS, 0.2M lithium sulfate, pH 10, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
