3CDK
Crystal structure of the co-expressed succinyl-CoA transferase A and B complex from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 69.337, 70.404, 97.996 |
| Unit cell angles | 90.00, 106.31, 90.00 |
Refinement procedure
| Resolution | 48.300 - 2.590 |
| R-factor | 0.19564 |
| Rwork | 0.193 |
| R-free | 0.25278 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m3e |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.599 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.147 | 0.543 |
| Number of reflections | 28084 | |
| <I/σ(I)> | 5.9 | 2.2 |
| Completeness [%] | 99.7 | 94.2 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.1M tri-Sodium citrate dihydrate pH 5.6, 20% Isopropanol, 20% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
