3CCH
H-2Db complex with murine gp100
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-03-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.500, 176.600, 85.500 |
| Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
| Resolution | 19.600 - 2.600 |
| R-factor | 0.24266 |
| Rwork | 0.241 |
| R-free | 0.27859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 65171 | |
| Completeness [%] | 96.4 | 89.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 1.8M Ammonium Sulfate, 0.1M Tris, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
