3CC6
Crystal structure of kinase domain of protein tyrosine kinase 2 beta (PTK2B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9753 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.450, 96.140, 43.200 |
| Unit cell angles | 90.00, 93.66, 90.00 |
Refinement procedure
| Resolution | 18.840 - 1.600 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2etm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.279 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.840 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.081 | 0.441 |
| Number of reflections | 40123 | 6650 |
| <I/σ(I)> | 16.22 | 4.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.05M Magnesium chloride, 0.1M Bis-tris, 17.5% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
