3CB6
Crystal Structure of the S. pombe Peptidase Homology Domain of FACT complex subunit Spt16 (form B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9699 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.980, 193.670, 89.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.850 - 1.840 |
| R-factor | 0.19371 |
| Rwork | 0.192 |
| R-free | 0.22472 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cb5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 1.950 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 47711 | |
| <I/σ(I)> | 20.49 | 4.1 |
| Completeness [%] | 99.6 | 98.4 |
| Redundancy | 7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20% PEG 300, 0.1M Mes, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
